| Culture |
| Organoid Research Core (ORC) | Web Form |
| The ORC provides long-term storage (-150 C) of organoids, training in organoid culture, compound screening, and consultation Location: Shadyside, FORD |
| Tissue Culture and Histology Core Facility | Katherine Davoli |
| "The Tissue Culture and Histology Core Facility within the Department of Ophthalmology offers support from tissue processing to H&E staining for FFPE or fresh frozen specimens. Basic and advanced tissue culture techniques are available. The core is open to all researchers with a vision-related project regardless of department or institutional affiliation. Training is required for user-operated access to all equipment or services can be performed by facility staff."
Location: Uptown, MERCY |
| Cytometry & Sorting |
| Cell Cytometry and Sorting Core at Magee-Women's Research Institute | Aaron Siegel |
| The Cell Cytometry and Sorting Core at Magee-Womens Research Institute provides the latest technology for cutting-edge flow cytometry research. The core facilities assist researchers in simultaneous, rapid examination of diverse individual cell types within a population of cells suspended in fluid. Location: Oakland, MWRI |
| Cytometry Facility, UPMC Hillman Cancer Center | E. Michael Meyer |
| The Cytometry Facility at UPMC Hillman Cancer Center supports analytical cytometry and cell sorting. The facility also offers NanoString Digital Spatial Analyzer and nCounter service and support. Location: Shadyside, FORD |
| Flow Cytometry Core at John G. Rangos Sr. Research Center | General Email |
| The Flow Cytometry Core at John G. Rangos Sr. Research Center is a BSL2+ compliant cytometry analysis and sorting facility located at the John G. Rangos Sr. Research Center in Lawrenceville serving investigators from across the University of Pittsburgh.
Location: Lawrenceville, RANCH |
| Flow Cytometry Facility at the McGowan Institute for Regenerative Medicine | Dewayne Falkner |
| The Flow Cytometry Facility at the McGowan Institute for Regenerative Medicine aims to make state-of-the-art flow cytometry available to all researchers across the Pittsburgh campus. The facility provides instrumentation and experienced staff to assist users in the understanding, development and usage of multiparameter flow cytometry in order to further their research aims. Location: Riverside, BRDG2 |
| Microscopy and Imaging Suite | Chaowei Shang |
| The Microscopy and Imaging Suite offers equipment and resources for light, confocal and electron microscopy. Flow cytometer is also available, as is software for image and cytometry analysis. Wet lab space is available for independent EM sample prep. Location: Oakland, LANG |
| Unified Flow Cytometry Core | Dewayne Falkner |
| The Unified Flow Cytometry Core provides extensive flow cytometry and cell sorting services with instruments in BST and at the Assembly. Instrument capabilities include traditional, spectral and imaging flow analysis as well as chip cytometry and cell sorting.
Location: Oakland, BST & Shadyside, FORD |
| Imaging |
| Cell and Tissue Imaging Facility, UPMC Hillman Cancer Center | Simon Watkins |
| The Cell and Tissue Imaging Facility at UPMC Hillman Cancer Center supports microscopy and imaging, including live cell, live tissue, and animal imaging. Location: Oakland, BSTWR-S |
| Center for Biologic Imaging | Simon Watkins |
| The Center for Biologic Imaging provides integrated elite level access and training on infrastructure used for research, education, technology development and collaboration in Microscopy, Biophotonics and Imaging Location: Oakland, BSTWR-S |
| Flow Cytometry Core at John G. Rangos Sr. Research Center | General Email |
| The Flow Cytometry Core at John G. Rangos Sr. Research Center is a BSL2+ compliant cytometry analysis and sorting facility located at the John G. Rangos Sr. Research Center in Lawrenceville serving investigators from across the University of Pittsburgh.
Location: Lawrenceville, RANCH |
| The Cell Imaging Core Laboratory at John G. Rangos Sr. Research Center | Krishna Prasadan |
| The Cell Imaging Core Laboratory at John G. Rangos Sr. Research Center offers cell imaging by confocal microscopy, including live cell as well as laser capture microdissection and LCM.
Location: Lawrenceville, RANCH |
| Unified Flow Cytometry Core | Dewayne Falkner |
| The Unified Flow Cytometry Core provides extensive flow cytometry and cell sorting services with instruments in BST and at the Assembly. Instrument capabilities include traditional, spectral and imaging flow analysis as well as chip cytometry and cell sorting.
Location: Oakland, BST & Shadyside, FORD |
| Single Cell |
| High Throughput Genome Center | Jianhua Luo, MD |
| The High Throughput Genome Center supports high throughput paralleled sequencing for genome, transcriptome and epigenome analyses through Illumina and Oxford nanopore platforms, and array through Affymetrix platform. In addition, the center provides collaboration on single-cell transcriptome and multi-omic sequencing. For spatial biology, we provide Cytassist and Stereo-Seq platforms for transcriptome analysis. The center also provides extensive bioinformatic analyses to investigators through collaboration. Location: Oakland, SCAIF |
| Organoid Research Core (ORC) | Web Form |
| The ORC provides long-term storage (-150 C) of organoids, training in organoid culture, compound screening, and consultation Location: Shadyside, FORD |
| Single Cell Core | Web Form |
| The Single Cell Core provides library preparation and analysis using the 10X Genomics Chromium platform. All 10X Genomics single cell applications are fully supported including gene expression, V(D)J B cell and T cell immune profiling, ATAC, Multiome (gene expression with ATAC), cell surface protein markers and FLEX (single cell processing of FFPE tissue sections and formaldehyde fixed cells and tissues). The Single Cell Core can also work with researchers to pilot other single cell technologies. Location: Oakland, BSTWR-S |
