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Searches can use "boolean" logic:

Prefixing a search term with a "+" denotes boolean "AND" operator.
Prefixing a search term with a "-" denotes boolean "AND NOT" operator.
Absence of a prefix is interpreted as "OR" operator.

Examples: ("Search term" => Logical interpretation)
"computing DNA" => "computing" OR "DNA"
"+computing +DNA" => "computing" AND "DNA"
"+computing -DNA" => "computing" AND NOT "DNA"

Wildcard "*" searches are also valid, but only at the end of a word.

"*" is interpreted as one or more characters.

Example:
"comput* +DNA" => "comput*" AND "DNA"

Phrases can be enclosed in double-quotes (")

Example:
""mass spec*" +proteomics" => "mass spec*" AND "proteomics"

All searches are case-insensitive.
Citations are an essential shared resource support. Acknowledge facilities as requested in papers, posters, and talks.
OMICS OMICS
Genomics
Cancer Genomics Facility, UPMC Hillman Cancer CenterMaureen Lyons
The Cancer Genomics Facility at UPMC Hillman Cancer Center supports genomics and transcriptomics platforms including nucleic acid extraction, sample QC, next-generation library preparation and sequencing, single cell and spatial analysis. The facility specializes in paraffin-embedded tissue samples. Location: Shadyside, SHDY Location: Shadyside, SHDY
Cytometry Facility, UPMC Hillman Cancer CenterE. Michael Meyer
The Cytometry Facility at UPMC Hillman Cancer Center supports analytical cytometry and cell sorting. The facility also offers NanoString Digital Spatial Analyzer and nCounter service and support. Location: Shadyside, FORD
Health Sciences Sequencing Core at Children's Hospital of PittsburghDr. Will MacDonald
The Health Sciences Sequencing Core (HSSC) at UPMC Children's Hospital of Pittsburgh offers full-service next generation sequencing; including RNA/DNA extraction, library generation and sequencing on Illumina NextSeq 2000 or MiSeq sequencers. Protocols are established to accept a wide range of input quantity and quality. The HSSC also specializes in spatial transcriptomics and offers full-service options for 10x Visium, 10x Xenium, and Curio Bioscience Slide-seq Location: Lawrenceville, RANCH
High Throughput Genome CenterJianhua Luo, MD
The High Throughput Genome Center supports high throughput paralleled sequencing for genome, transcriptome and epigenome analyses through Illumina and Oxford nanopore platforms, and array through Affymetrix platform. In addition, the center provides collaboration on single-cell transcriptome and multi-omic sequencing. For spatial biology, we provide Cytassist and Stereo-Seq platforms for transcriptome analysis. The center also provides extensive bioinformatic analyses to investigators through collaboration. Location: Oakland, SCAIF
High Throughput Genomics CoreWeb Form
The High Throughput Genomics Core (HTGC) for clinical and translational medicine offers Illumina genome and exome sequencing using a semiautomated CAP compliant platform. Location: Shadyside, 5560 Centre Ave.
Single Cell CoreWeb Form
The Single Cell Core provides library preparation and analysis using the 10X Genomics Chromium platform. All 10X Genomics single cell applications are fully supported including gene expression, V(D)J B cell and T cell immune profiling, ATAC, Multiome (gene expression with ATAC), cell surface protein markers and FLEX (single cell processing of FFPE tissue sections and formaldehyde fixed cells and tissues). The Single Cell Core can also work with researchers to pilot other single cell technologies. Location: Oakland, BSTWR-S
Metabolomics
Health Sciences Mass Spectrometry CoreGeneral email
The Health Sciences Mass Spectrometry Core provides liquid chromatography high resolution mass spectrometry (LC-HRMS) services for metabolomics, lipidomics and proteomics. We also provide proteomics analysis using the O-link midplex platform. Expert staff provide assistance throughout the full lifespan of all projects from experimental design and sample preparation through statistical analysis and interpretation of datasets. Location: Oakland, BST3
OMICS Data Analysis
Bioinformatics Core Facility at the John G Rangos Sr. Research CenterDhivyaa Rajasundaram
The Bioinformatics Core Facility at the John G. Rangos Sr. Research Center provides bioinformatics as well as biostatistics services across a wide range of disease domains. The next-generation sequencing data analysis includes, but is not limited to, whole genome-, whole exome-, bulk and single cell epigenome-, bulk and single cell transcriptome, spatial transcriptome, microbiome, metabolome, proteome and lipidome data analysis. The facility specializes in T-, and B- cell repertoire data analysis, multi-omics initiatives, and multimodal analysis. Shiny dashboard and other web application development is also supported. Location: Lawrenceville, RANCH
Cancer Bioinformatics Services, UPMC Hillman Cancer CenterRiyue Bao or Uma Chandran
The Cancer Bioinformatics Services at UPMC Hillman Cancer Center provides bioinformatics support for translational genomics using sequencing, arrays, single cell, and other technologies. Location: Shadyside, HCC
Genomics Analysis CoreUma Chandran
The Genomics Analysis Core focuses on bioinformatics analysis of genomic data with emphasis on rigor and reproducibility. Professional analytical staff support all aspects of translational studies - from experimental design to data analysis to publication. Location: Shadyside, BAUM
Health Sciences Library System Molecular Biology Information ServiceAnsuman Chattopadhya
The Health Sciences Library System Molecular Biology Information Service office maintains institutional licenses to a number of analytical software tools. Training workshops are offered regularly on how to use many of those packages, including those used for RNA-Seq and single-cell transcriptomics analysis. Location: Oakland, SCAIF
High Throughput Genome CenterJianhua Luo, MD
The High Throughput Genome Center supports high throughput paralleled sequencing for genome, transcriptome and epigenome analyses through Illumina and Oxford nanopore platforms, and array through Affymetrix platform. In addition, the center provides collaboration on single-cell transcriptome and multi-omic sequencing. For spatial biology, we provide Cytassist and Stereo-Seq platforms for transcriptome analysis. The center also provides extensive bioinformatic analyses to investigators through collaboration. Location: Oakland, SCAIF
Proteomics
Cytometry Facility, UPMC Hillman Cancer CenterE. Michael Meyer
The Cytometry Facility at UPMC Hillman Cancer Center supports analytical cytometry and cell sorting. The facility also offers NanoString Digital Spatial Analyzer and nCounter service and support. Location: Shadyside, FORD
Health Sciences Mass Spectrometry CoreGeneral email
The Health Sciences Mass Spectrometry Core provides liquid chromatography high resolution mass spectrometry (LC-HRMS) services for metabolomics, lipidomics and proteomics. We also provide proteomics analysis using the O-link midplex platform. Expert staff provide assistance throughout the full lifespan of all projects from experimental design and sample preparation through statistical analysis and interpretation of datasets. Location: Oakland, BST3
Single Cell CoreWeb Form
The Single Cell Core provides library preparation and analysis using the 10X Genomics Chromium platform. All 10X Genomics single cell applications are fully supported including gene expression, V(D)J B cell and T cell immune profiling, ATAC, Multiome (gene expression with ATAC), cell surface protein markers and FLEX (single cell processing of FFPE tissue sections and formaldehyde fixed cells and tissues). The Single Cell Core can also work with researchers to pilot other single cell technologies. Location: Oakland, BSTWR-S
Spatial Biology
Cancer Genomics Facility, UPMC Hillman Cancer CenterMaureen Lyons
The Cancer Genomics Facility at UPMC Hillman Cancer Center supports genomics and transcriptomics platforms including nucleic acid extraction, sample QC, next-generation library preparation and sequencing, single cell and spatial analysis. The facility specializes in paraffin-embedded tissue samples. Location: Shadyside, SHDY Location: Shadyside, SHDY
Cytometry Facility, UPMC Hillman Cancer CenterE. Michael Meyer
The Cytometry Facility at UPMC Hillman Cancer Center supports analytical cytometry and cell sorting. The facility also offers NanoString Digital Spatial Analyzer and nCounter service and support. Location: Shadyside, FORD
Health Sciences Sequencing Core at Children's Hospital of PittsburghDr. Will MacDonald
The Health Sciences Sequencing Core (HSSC) at UPMC Children's Hospital of Pittsburgh offers full-service next generation sequencing; including RNA/DNA extraction, library generation and sequencing on Illumina NextSeq 2000 or MiSeq sequencers. Protocols are established to accept a wide range of input quantity and quality. The HSSC also specializes in spatial transcriptomics and offers full-service options for 10x Visium, 10x Xenium, and Curio Bioscience Slide-seq Location: Lawrenceville, RANCH
High Throughput Genome CenterJianhua Luo, MD
The High Throughput Genome Center supports high throughput paralleled sequencing for genome, transcriptome and epigenome analyses through Illumina and Oxford nanopore platforms, and array through Affymetrix platform. In addition, the center provides collaboration on single-cell transcriptome and multi-omic sequencing. For spatial biology, we provide Cytassist and Stereo-Seq platforms for transcriptome analysis. The center also provides extensive bioinformatic analyses to investigators through collaboration. Location: Oakland, SCAIF
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